Psiwdomonas Cjleoi/orans as a Nonheme Iron Protein Requiring Phospholipid for Catalytic Activity”
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چکیده
Fatty acid and alkane hydroxylation by the inducible, cyanide-sensitive enzyme system of Pseudomonas oleovorans involves three enzymes : NADH-rubredoxin reductase, rubredoxin, and an w-hydroxylase. The w-hydroxylase is a yellow protein containing one atom of iron per polypeptide chain of molecular weight 42,000. Catalytic activity is lost upon removal of the iron and is restored when ferrous ions are incubated with the apohydroxylase. Hydroxylase preparations from which most of the phospholipid has been removed exhibit decreased activity unless supplemented with a phospholipid fraction from this bacterium or with dilauroylglyceryl3-phosphorylchol ine.
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تاریخ انتشار 2003